Coding

Part:BBa_K615001:Design

Designed by: Naomi Genuth   Group: iGEM11_Harvard   (2011-09-24)


Zinc finger-omega subunit fusion protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 270
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 309
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Array designed to bind to 5'-GTGGGATGG-3', of which the first six bases represents a known CoDA pair while no binders have yet been found for the last codon in the context of the other fingers.


Source

CoDA sequences from: Jeffry D Sander, Elizabeth J Dahlborg, Mathew J Goodwin et al. Selection-free zinc-finger-nuclease engineering by contextdependent assembly (CoDA). (2011) Nature Methods 8(1): 53-55.

References